WebFeb 1, 2024 · Differential epitope mapping saturation transfer difference (DEEP‐STD) NMR spectroscopy is a recently developed powerful approach for elucidating the structure and pharmacophore of weak protein–ligand interactions, as it reports key information on the orientation of the ligand and the architecture of the binding pocket.1 The method relies on … WebNov 18, 2011 · Saturation transfer difference NMR of Q 1 interacting with NqrA. Upper and lower panels, reference (upper panel) and STD NMR (lower panel) spectra of Q 1 in the presence of NqrA (molar ratio = 10:1). The reference spectrum was scaled to 10% of its original intensity. Signals of the same intensity in the STD spectrum corresponded to an …

Use of NMR saturation transfer difference spectroscopy to study …

WebWith an attenuation of 50 dB, the radio frequency field strength for the selective saturation pulses in all STD NMR experiments was 190 Hz. The frequency of the protein (on … WebOct 14, 2024 · All the STD-NMR experiments were performed on Bruker 600 MHz NMR instrument at 298 K using Stddiffesgp.3 pulse program Saturation time was 3 s, while interpulse delay (D1) was the same as D20 or D20 + 1 [ 40 ]. The loop counter was 8.0 and 4.0. STD-NMR spectra were recorded with 1024 scans (NS). how to track standard mail

Glycosylation Assists Binding of HIV Protein gp120 to Human CD4 ...

WebSTD NMR is a powerful ligand-based tool for screening small molecules and low molecular weight fragments for their interaction with a given macromolecule. Such information is … WebJan 1, 2008 · Prepare an identical STD NMR sample (as for sample A2 or B2) but in the absence of the protein. PeForm a STD NMR experiment using the same parameters as selected for the original STD NMR experiment. As no protein is present in the NMR sample, no saturation transfer occurs and hence the obtained final STD NMR spectrum reveals no … how to track sprint cell phone