Phic31 integrase
WebFinally, the serine integrase PhiC31 was also applied in the yeast Saccharomyces cerevisiae as a marker recovery tool, indicating the … WebOct 17, 2005 · The Streptomyces phage phiBT1, a homoimmune relative of phiC31, integrates its genome into a distinct site in the genome of Streptomyces coelicolor. The phiBT1 integrase is also a member of the large serine recombinase family, and although it shares many organizational features with phiC31, there is no significant similarity …
Phic31 integrase
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WebPhiC31 integrase, a site-specific large serine recombinase, is a useful tool for genome engineering in a variety of eukaryotic species and cell types. PhiC31 integrase performs efficient recombination between its attB site and either its own placed attP site or a partially mismatched genomic pseudo attP site. WebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ...
Webbacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 base pair sequences termed attachment sites (att), one found in the phage and the other in the … http://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf
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WebThe phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also mediates integration …
WebThe Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. … f\u0026g tool dayton ohioWebPhiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. SBI’s PhiC31 … f\u0026g sports cards elkhart inWebJul 25, 2024 · A master cell line, with the landing pad inserted correctly in the H11 locus, was established. This master cell line was used for site-specific, irreversible recombination, catalyzed by PhiC31 integrase. Using this system, an integration efficiency of 97.7% was achieved with green fluorescent protein (GFP) after selection. gilliam arkansas is what countyWebOct 28, 2024 · In one exemplary embodiment, the method disclosed herein selects a variant integrase (e.g., a phiC31 integrase) that can recognize a mutant attP sequence (e.g., SEQ ID NO: 3). The target mutant attP sequence is different from the wildtype attP site in more than 50% of the nucleotide residues, e.g., 26 out of 48 nucleotide residues are different. gilliam and sons landscapingWebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site ( attB) and a phage attachment site ( attP ). f \u0026 h contractors floridaWebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. gilliam and bisbee heppner orhttp://www.hbaas.com/xumusuo/view/15912.html gilliam asperger\\u0027s test