Phic31 integrase vector system
WebA polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on phiC31 integrase supplies a simple method to obtain repeated integration at the same chromosomal site in mammalian cells. Publication types Research Support, N.I.H., Extramural MeSH terms WebJun 1, 2024 · Our results demonstrated that dual ORF vector in the presence of phiC31 integrase expression vectors (+FC31 2P) generated more recombinant antibody in comparison to its negative control (-FC31 2P). Moreover, both of +FC31 2P and -FC31 2P cell pools yield higher recombinant protein in comparison to single ORF/IRES vector (FC31 …
Phic31 integrase vector system
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WebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An …
WebThe PhiC31 Integrase System Popularly used for gene therapy development and related applications, one of the major advantages of SBI’s PhiC31 system is the tight control over … WebJun 1, 2024 · Co-transfection of dual ORF vector with PhiC31 integrase expression vector resulted in FC31 2P cell pools. As the negative control, cells transfected just with single-ORF/IRES or dual-ORF vectors, without phiC31 integrase expression vector. A total amount of 3 μg plasmid DNA was used for each well of the 6-well plate.
WebApr 10, 2024 · The pUASTattB system consists of two vectors, both engineered as E. coli plasmids. One vector referred to as the pUASTattB vector or the φC31 donor vector carries the attB site and the user’s gene of interest. The other vector referred to as the helper plasmid encodes for the φC31 integrase. WebPhiC31 integrase [ edit] Without much doubt, Ser integrases are the current tools of choice for integrating transgenes into a restricted number of well-understood genomic acceptor …
Webprotein (EGFP) positive and phiC31 integrase could recombine the reporter gene in a site specific manner. These results demonstrate that attP site can be used as a proper location to conduct site directed transgenesis in both mammalian cells and embryos in phiC31 integrase system when even combinaed to SCNT and intracytoplasmic sperm
WebJun 1, 2024 · In this study, we have compared the mAb0014 expression level of single ORF/IRES vector and dual ORF vector in the presence and absence of phiC31 integrase … glossier cloud paint bundle: dawn + sparkWebIn addition, with the recent creation of a hybrid LV combining the NILV for gene delivery and a site-specific integrase (phiC31-int) for specific integration (Grandchamp et al., 2014), the ... glossier clear brow gelWebIn this study, phiC31 integrase technology in combination with DHFR amplification system was used to have an expression vector for future expression of blinatumomab in CHO cells. The gene of interest (BsAb gene) could be joined to DHFR selection marker with the insertion of an internal ribosome entry site (IRES). glossier cloud paint blush beamWebMay 12, 2014 · Vector constructs used in this study. (A and B) The genetic structure of the barnase expression vector (“pro-vector”) (A) and the phiC31-integrase-expression vector (B). The provector harbors attP and attB sequences that serve as targets for the site-specific recombinase phiC31. Note that only the T-DNA part of the vectors is illustrated ... glossier clear lip balmWebDec 9, 2024 · This vector system consists of several modules including a Tet-inducible promoter directing the expression of a transgene and two Tet repressor expression units placed in tandem on a single vector. The cell clones, generated using a one-step phiC31 integrase-mediated chromosomal integration of the multi-cDNA expression construct, … boid翻译WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … boi dividend historyWebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Contact Us - PhiC31 Integrase Expression Plasmid System Biosciences Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … NTA-based Exosome Characterization - PhiC31 Integrase Expression Plasmid … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … CLOuD9 Gene Expression Regulation System; Targeted Protein Degradation; … Lentiviral Packaging Kits - PhiC31 Integrase Expression Plasmid System Biosciences How it works. Ordering RNA Services is easy. Step 1: Contact the services team … Exosome Biomarker Discovery Products - PhiC31 Integrase Expression Plasmid … Get everything you need for studying and using exosomes, from isolation and … boid rated