Phic31 integrase system
WebJul 29, 2012 · Notably, a phiC31 integrase mRNA synthesized from two of the plasmids produced cassette exchange plasmids at a high frequency, suggesting that the mRNA can be used to construct a targeted integration system in silkworms. Introduction Transgenic organisms are very useful tools to analyze gene function. WebFeb 23, 2010 · The phiC31 system performs site-specific recombination in germinal tissue, a prerequisite for generating stable lines with unwanted DNA removed. ... Weyen J, Gils M: Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants. Plant Cell Rep. 2008, 27: 1821-1831. 10.1007/s00299-008-0604-z. Article CAS Google Scholar ...
Phic31 integrase system
Did you know?
WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two … WebMay 20, 2012 · It is shown that PhiC31 integrase can mediate site-specific transgene integration into the genome of pig kidney PK15 cells and form the basis for targeted pig genome engineering and may be used to produce genetically modified pigs for agricultural and biomedical uses. 13 PDF
WebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, …
WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non …
WebSimply co-transfect with the PhiC31 Integrase Expression Plasmid (Cat.# FC200PA-1) and select for integrants using puromycin resistance and imaging for RFP fluorescence. The bidirectional promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter.
WebThe Streptomyces phage phiC31 uses a large serine recombinase, phiC31 integrase (Int), to merge its genome with that of its bacterial host [ 1, 2 ]. Its two substrates – attP and attB – are distinct (only ~40% identical, Supporting Information, Table S1) [ 2 ]. cliff hill music orderWebWe developed integrase-mediated site-specific transgenesis in mice via pronuclear microinjection. We use PhiC31 integrase to catalyze recombination between one or two … cliff hill musicWebPlasmid pcDNA3.1 phiC31 from Dr. Konrad Basler's lab contains the insert phiC31 and is published in Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7. ... integrase Alt name. serine family SSR phiC31 integrase Insert Size (bp) ... An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. board ghostsWebThe φC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The φC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of the integrated transgene. This review describes the nature of the integration reaction and … board geral - sakata – planner office.comWebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … board geometry panelWebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... board geometry subclassesWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 cliff hill music inc