Web5 mrt. 2024 · Solene Accarias, Thibaut Sanchez, Arnaud Labrousse, Myriam Ben-Neji, Aurélien Boyance, Renaud Poincloux, Isabelle Maridonneau-Parini, Véronique Le Cabec, Ana-Maria Lennon-Duménil; Genetic engineering of Hoxb8-immortalized hematopoietic progenitors – a potent tool to study macrophage tissue migration. Web- HoxB8: nuclear homeobox protein that suppresses myeloid cell development - immortalization of bone marrow myeloid progenitors by HoxB8 overexpression - Retrovirus transduction of HoxB8 gene driven by estrogen receptor followed by selection in b-estradiol and SCF (Wang et.al, Nature Methods, 2006)
Single Cell RNA-Seq Characterises Pre-Leukemic Transformation …
Web26 nov. 2024 · The HoxB8 MPP differentiation system developed here extends previous work on HoxB8 cells and DC differentiation [6, 9 – 11] and generates the full complement of DC subsets, cDC1, cDC2, and pDC. In addition, we established a versatile workflow for gene targeting with CRISPR/Cas9 to study DC differentiation. Web29 nov. 2024 · Serial monitoring of peripheral blood demonstrated that Hoxb8-FL derived cells had disappeared by 4 weeks, and did not reappear. However, at 6 months CEBPA N321D-transduced cells could still be detected in bone marrow in contrast to EV-transduced cells but without any leukemic phenotype. spiral of ants
GEO Accession viewer - National Center for Biotechnology …
Web15 nov. 2024 · HoxB8-FL cells expressing Myb-FL, Myb-TR or Myb-PLEKHO1 displayed arrested differentiation upon withdrawal of E2 in vitro (Figure 1). While all empty vector-transduced cells had upregulated CD11b and/or CD11c at day 7 post-E2 withdrawal, Myb overexpression caused an accumulation of CD11b-CD11c-undifferentiated cells. In vivo, … WebTreatment of ERHBD-Hoxb8-expressing progenitors with estrogen results in Hoxb8 activation, which blocks cell differentiation and allows for expansion of homogenous progenitor cell populations in the presence of FLT3L. These cells, referred to as Hoxb8-FL cells, retain lineage potential for lymphocyte and myeloid lineages, including the DC … spiral of fire