WebThe blot was probed with Anti-B7-H3 Monoclonal Antibody (6A1) (Product # MA5-15693, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 100 kDa band corresponding to B7-H3 was detected in THP-1 differentiated … WebAdd no more than 15 µg of chromatin and 10 µl of histone H3 antibody to each IP reaction. Reduce the amount of normal rabbit IgG to 1 µl per IP. Add less DNA to the PCR reaction or decrease the number of PCR cycles. It is very important that the PCR products are analyzed within the linear amplification phase of PCR.
B7-H3 Monoclonal Antibody (6A1) (MA5-15693) - Thermo Fisher Scientific
WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment ... WebImmunocytochemistry analysis of Histone H3ac (pan-acetyl) in HeLa cells (4h of LPS treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody ( Product # PA5-114693) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse ... tradicionalna hrana v grčiji
Histone H3 Antibody (ChIP Formulated) - Cell Signaling …
WebHistone H3ac (pan-acetyl) antibody (pAb) tested by Western blot. Detection of acetylated Histone H3 by Western blot analysis using 10 µg HeLa acid extracts and Histone H3 acetyl antibody at a 1:2,000 dilution. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate. WebHuman bone marrow mesenchymal stem cell-derived extracellular vesicles reduce inflammation and pyroptosis in acute kidney injury via miR-223-3p/HDAC2/SNRK. In Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.] on 1 March 2024 by Xie, Z., Tang, J., et al.. WebAntibodies that detect B7-H3 (CD276) can be used in several scientific applications, including ELISA, Western Blot, Immunohistochemistry, Flow Cytometry and Immunocytochemistry. These antibodies target B7-H3 (CD276) in Human, Mouse, Rat and Porcine samples. Our B7-H3 (CD276) monoclonal, polyclonal and recombinant … tradicionalna bosanska jela recepti